N1-Methylpseudo-UTP

N1-Methylpseudo-UTP

Author: ApexBio

purity:

≥ 90% by AX-HPLC

shortsummary:

A nucleobase-modified nucleotide, used for drugable mRNA synthesis

halt protease inhibitor cocktail

Synonyms:

N1-Methylpseudouridine-5'-Triphosphate, 1-Methylpseudo-UTP

Protease Inhibitor Cocktail for bacterial cell extracts

Formula:

C10H17N2O15P3 (free acid)

M.Wt:

498.1 (free acid)

Chemical Name:

lithium (3,4-dihydroxy-5-(1-methyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)tetrahydrofuran-2-yl)methyl triphosphate

SMILES:

O=C(N(C)C=C1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+]

Storage:

Store at -20°C or below

Physical Appearance:

Solution

DeliveryTime:

In stock

protease phosphatase inhibitor cocktail

ShippingType:

Dry ice

description:

N1-methylpseudo-UTP is a nucleobase-modified nucleotide, used for synthesizing mRNA with reduced immunogenicity and improved stability. It was reported that N1-methylpseudouridine (m1Ψ) modification alone or in combination with 5-methylcytidine (m5C) exhibited superiority over the current state-of-the-art pseudouridine (Ψ) or m5C/Ψ-modified mRNA platform by providing up to ~ 44-fold (when comparing double modified mRNAs) and ~ 13-fold (when comparing single modified mRNAs) higher reporter gene expression in cells and mice, respectively. Moreover, compared with (m5C/)Ψ-modified mRNAs, (m5C/)m1Ψ-modified mRNAs showed reduced intracellular innate immunogenicity and resulted in improved cellular viability after in vitro transfection. Thus, N1-methylpseudo-UTP might serve as a useful ingredient for synthesizing drugable mRNAs with better performance.

related websites:

APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 7209 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.